Resumen

PAZ DA SILVA, Raquel  y  VILLEGAS MONTER, Ángel. Sucrose levels in the in vitro rooting and ex vitro acclimatization of plantlets of the R110 (vitis rupestris x vitis berlandieri) grapevine rootstock. INCI [online]. 2009, vol.34, n.12, pp.897-902. ISSN 0378-1844.

Sucrose concentration and container gas exchange are factors that influence the characteristics of micropropagated and acclimatized plantlets. Apical buds of the rootstock of the R110 (Vitis rupestris´Vitis berlandieri) grapevine were cultivated in media containing 0, 43.8, 87.6, 131.4, and 175.2mM sucrose, in containers with or without complementary gas exchange. After 13 days of growth, the plantlets were transplanted and placed in a greenhouse, where they remained for nine weeks. Percentage of rooting, number and length of the roots, bud size, weight of fresh material and water content in vitro, all decreased with the increase in the concentration of sucrose, the change being more pronounced in containers with gas exchange. In the acclimatization stage, sucrose concentration and type of container did not influence the number and length of the roots. However, bud size, number of leaves, and water content were lower in the plantlets growing with 175.2mM sucrose in containers with gas exchange. The survival rate was 100% after nine weeks with 43.8mM of sucrose in containers without exchange, and with 43.8 and 87.6mM in containers with gaseous exchange.

Palabras clave : Adaptación ex vitro; Contenedor; Fuente de Carbono; Intercambio Gaseoso; Micropropagación; Vitis.