Abstract

CALVO, Jorge; MORRICONI, Elba R  and  BOY, Claudia C. An alternative method to freeze and transport biological tissue blocks for microscopy. INCI [online]. 2008, vol.33, n.3, pp.223-224. ISSN 0378-1844.

A cheap and simple method for freezing raw biological material destined to obtain histological sections through a cryostat is described. We propose the utilization of solid CO₂ (dry ice) to make a cooling mixture, instead of liquid nitrogen. The temperature of the refrigerating mixture (composed by acetone and dry ice) reaches isopentane to -76ºC which is maintained for ~1.5h allowing the freezing of more than 20 tissue blocks. The quality of cryostat sections obtained is similar to the ones obtained by using liquid nitrogen. This method is particularly suitable during sampling on board, and/or far away from urban centers

Keywords : Histología; CO₂; Método de Congelamiento; Secciones de Crióstato.