<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0535-5133</journal-id>
<journal-title><![CDATA[Investigación Clínica]]></journal-title>
<abbrev-journal-title><![CDATA[Invest. clín]]></abbrev-journal-title>
<issn>0535-5133</issn>
<publisher>
<publisher-name><![CDATA[Instituto de Investigaciones Clínicas "Dr. Américo Negrette", Facultad de Medicina, Universidad del Zulia]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0535-51332023000100005</article-id>
<article-id pub-id-type="doi">10.54817/ic.v64n1a01</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Correlation between bacterial type/bacterial quantity and bone loss detected by cone beam computed tomography (CBCT) in primary endodontic infections.]]></article-title>
<article-title xml:lang="es"><![CDATA[Correlación entre el tipo y cantidad de bacterias y la pérdida ósea detectada por Tomografía Computarizada de Haz Cónico (CBCT) en infecciones endodóncicas primarias.]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Oliva Rodríguez]]></surname>
<given-names><![CDATA[Ricardo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rangel Galván]]></surname>
<given-names><![CDATA[Georgina Yaretzy]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[González Amaro]]></surname>
<given-names><![CDATA[Ana María]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Gutiérrez Cantú]]></surname>
<given-names><![CDATA[Francisco Javier]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Muñoz Ruiz]]></surname>
<given-names><![CDATA[Abraham Israel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[García Cortés]]></surname>
<given-names><![CDATA[José Obed]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Mariel Cárdenas]]></surname>
<given-names><![CDATA[Jairo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Autónoma de San Luis Potosí Facultad de Estomatología Programa de Postgrado en Endodoncia]]></institution>
<addr-line><![CDATA[San Luis Potosí SLP]]></addr-line>
<country>México</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Autónoma de San Luis Potosí Facultad de Estomatología Programa de Postgrado en Ciencias Dentales]]></institution>
<addr-line><![CDATA[San Luis Potosí SLP]]></addr-line>
<country>México</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>03</month>
<year>2023</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>03</month>
<year>2023</year>
</pub-date>
<volume>64</volume>
<numero>1</numero>
<fpage>5</fpage>
<lpage>14</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://ve.scielo.org/scielo.php?script=sci_arttext&amp;pid=S0535-51332023000100005&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://ve.scielo.org/scielo.php?script=sci_abstract&amp;pid=S0535-51332023000100005&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://ve.scielo.org/scielo.php?script=sci_pdf&amp;pid=S0535-51332023000100005&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Periapical lesions (PL) of endodontic origin are one of the most common pathological conditions that affect peri-radicular tissues. The main objective of this study was to evaluate the amount and species of microorganisms isolated from necrotic pulps, establish a correlation between these and the size of periapical lesions, and how the amount and species of microorganisms decreased with non-surgical root canal treatment. Twenty-seven patients with a clinical diagnosis of dental pulp necrosis and chronic periapical lesions were selected; a Cone Beam Computed Tomography (CBCT) and microbial samples of the root canal system were taken previous to a disinfection protocol, a post-instrumentation/ disinfection protocol, and a post-medication placement. Samples were processed for colony-forming unit (CFU) counting, Gram staining technique, and bacterial identification by the API-20 Strep/API-20A system. The API system identified 21 species of microorganisms in the pre-instrumentation samples, 11 species in the post-instrumentation samples, and 11 in the post-medication samples. There was a correlation coefficient of 0.598% between the initial size of the lesion and the number of bacteria, with a coefficient of determination up to 35.7%, a correlation coefficient of 0.486% and a determination coefficient of 23.6% between the size of the periapical lesion and the number of CFUs. This study contributes to the knowledge of the amount and species of microorganisms isolated and identified from necrotic pulps, establishes a correlation between the amount and species of microorganisms and the size of the periapical lesions, and shows how the decrease of microorganisms contributes to the healing of PL, corroborating the importance of an adequate disinfection protocol.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Las lesiones periapicales (LP) de origen endodóncico son la condición patológica más común que afectan los tejidos perirradiculares. El objetivo principal de este estudio es evaluar la cantidad y especie de bacterias aisladas de pulpas necróticas, correlacionar la cantidad y especies bacterianas con el tamaño de la lesión, y cómo disminuyen la cantidad y especies de microorganismos con el tratamiento de conductos. A 27 pacientes con diagnóstico de necrosis pulpar y lesión periapical crónica detectada con CBCT se les tomaron muestras microbianas del sistema de conductos antes y después del protocolo de desinfección y de la medicación intraconducto. Las muestras se procesaron para el recuento de unidades de formación de colonias (UFC), tinción de Gram e identificación mediante el sistema API-20 Strep/API-20A. Se identificaron 21 especies en las muestras pre-instrumentación, 11 en las muestras post-instrumentación y 11 en las muestras post-medicación; se observó un coeficiente de correlación del 0,598% entre el tamaño inicial de la lesión y la cantidad de bacterias, con un coeficiente de determinación hasta el 35,7%, un coeficiente de correlación del 0,486% y un coeficiente de determinación del 23,6% entre el tamaño de la lesión periapical y el número de UFCs. Este estudio contribuye al conocimiento sobre la cantidad y especies de microorganismos aislados e identificados a partir de pulpas necróticas, establece una correlación entre la cantidad y especies de microorganismos y el tamaño de las lesiones periapicales y exhibe cómo la disminución de microorganismos contribuye a la curación de LP, corroborando la importancia de un adecuado protocolo de desinfección.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[bone loss]]></kwd>
<kwd lng="en"><![CDATA[cone beam]]></kwd>
<kwd lng="en"><![CDATA[primary endodontic infection]]></kwd>
<kwd lng="es"><![CDATA[pérdida ósea]]></kwd>
<kwd lng="es"><![CDATA[haz cónico]]></kwd>
<kwd lng="es"><![CDATA[infección endodóncica primaria]]></kwd>
</kwd-group>
</article-meta>
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