Abstract

RODRIGUEZ-PACHECO, Javier E; PULIDO-MEDELLIN, Martín O  and  GARCIA-CORREDOR, Diego J. In vitro Resistance of Rhipicephalus microplus ticks to Organophosphates, Pyrethroids and Amitraz in Boyacá Department, Colombia. Rev. Fac. Cienc. Vet. [online]. 2017, vol.58, n.1, pp.17-23. ISSN 0258-6576.

The present investigation evaluated the in vitro resistance of the Rhipicephalus microplus tick, to organophosphates (OP), synthetic pyretroids (SP), and amitraz. Adult ticks were collected in the Department of Boyacá, Colombia, and subjected to the immersion test, to assess the efficacy of drugs, their effect on oviposition, and the percentage of larvae eclosion. A completely randomized experimental design was used, with 5 groups and 3 replicates per group, with 10 ticks per replicate. The results showed that the mortality rates in terms of percentage (%) of the assessed molecules were: 43 for the control group; 56 for SP; 60 for AM, and 96 for OP, respectively, at day 21 of the study. The inhibition of oviposition was: 13.4% for the control group; 44.6% for AM; 45.5% for SP; and 96% for OP. The present in vitro study represents the first diagnostic of resistance in the Department of Boyacá, Colombia.

Keywords : Rhipicephalus microplus; resistance; acaricides; in vitro.

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