Abstract

AGUILERA, Natalia; SILVA, Elizabeth  and  QUINONEZ, Belkis. Niveles extracelulares de glutamato y aspartato en el fluido gingival en la periodontitis.. Acta odontol. venez [online]. 2007, vol.45, n.2, pp.166-171. ISSN 0001-6365.

The objective of this work was to compare glutamate and aspartate levels in periodontal chronic localized disease (PCIP) and dental zones with gingivitis (GIP) in the gingival crevicular fluid (GCF). Periodontal inflammation produces histological changes, increase of blood irrigation and also increase of subgingival fluid. GCF was recognized as an inflammatory exudes derived from the periodontal tissue. Different methods to collect and analyze GCF samples had been used to identify some substances in the GCF, such as the proteinglycans metabolite, to be a possible marker of active periodontal disease. A combination of microdialysis in situ in dental zones with PCIP (probing depth ≥ 3 mm; attachment level ≥ 2 mm) and dental zones with GIP (n=10), total samples: 100, and capillary zone electrophoresis coupled to a laser induced fluorescence detection (CZE-LIFD) was used to measure extracellular concentrations of amino acids: glutamate and aspartate in the GCF in adult patients The results showed that glutamate decrease (p<0.05) and aspartate increase (p<0.02) in PCIP disease zones compared with dental zones with GIP. We observed chemical in vivo evidence that differentiate the GIP zones and PCIP zones.

Keywords : periodontitis; glutamato; aspartato; electroforesis capilar; fluido gingival.