Abstract

DO NASCIMENTO, Cássio; ISSA, João Paulo Mardegan  and  CATIRSE, Alma Blásida Concepción Elizaur. Use of checkerboard dna-dna hybridization technique for periimplantitis microbiota identification. Acta odontol. venez [online]. 2008, vol.46, n.3, pp.397-400. ISSN 0001-6365.

The periimplantitis term is characterized as a disease that affects the support implant tissues. It has a multifactorial etiology, with an important role of the biofilm on the periodontal diseases. The culture techniques use possibilited to identify bacterias responsible for periodontal alterations, as Fusobacterium, Prevotella and Porphyromonas ssp. The development of the molecular biology techniques possibilited the identification of some bacterial species, that there were not related in the literature and there were responsible for periodontal pathogenesis. The microbiota present on the periodontal pockets in overdentures may contribute in the orientation of the therapeutic procedures, considering that the structural alterations on the periodontal tissues are straightly related to microorganism presence, and it constitutes one of the causes that affect the oral rehabilitation treatment with implants. This, the aim of this study was to present a literature review of DNA-DNA hybridization technique in the identification of the microorganisms more related to periimplantitis.

Keywords : DNA Checkerboard; periimplantar microbiota; periimplantitis; osseointegrated implants.