Resumen

PARDI, Germán; GUILARTE, Carolina; CARDOZO, Elba Inés  y  BRICENO, Elsi Natalí. "Detección de enterococcus faecalis en dientes con fracaso en el tratamiento endodóntico". Acta odontol. venez [online]. 2009, vol.47, n.1, pp.110-121. ISSN 0001-6365.

Enterococcus faecalis is a coccal-shaped, Gram positive, facultative anaerobic and non motile bacteria. In recent years, several researchers have been interested in E. faecalis because this species has been identified like a frequent microorganism in endodontic treatment with failure. E. faecalis can growth and survive in presence of calcium hydoxide. Resistant capacity of E. faecalis to calcium hydroxide into root-filled teeth results in proliferation of this bacteria and subsecuently colonization and infection of root canal. The aim of this study was detect and identify this microorganism in teeth with failure in endodontic treatment. Twenty (20) root-filled teeth with guttapercha points and calcium hydroxide cement were selected. These teeth had endodontic tratments with failure. Control group was conformed by twenty (20) pulpar and/or periapical infection teeth without endodontic treatment. Samples of root canals were taken using sterile paper points, inoculated in tioglycolate broth media and growth obtained in this media were transferred to Agar Enterococcus plates and incubated at 37ºC for 72 hours in microaerofilical conditions. Identification of E. faecalis was possible by using API Rapid Strep identification kit. E. faecalis was isolated en 12 out of 20 teeth (60%) with endodontic failure, and also was found in 5 out of 20 teeth (25%) with pulpar and/or periapical infection. These findings relected the high frequency that E. faecalis was detected and identified in root-filled teeth with endodontic failure.

Palabras clave : Enterococcus faecalis; teeth; endodontic treatment; failure.