Resumen

ARAMBURU, Guillermo; AGUZZI, Alejandra; HUBERT, Sergio  y  VIRGA, Carolina. Estudio histopatológico sobre el efecto de una nueva formulación de alendronato administrado por vía subcutánea. Acta odontol. venez [online]. 2009, vol.47, n.3, pp.60-62. ISSN 0001-6365.

Previous studies have shown that aminobisfosfonatos, such as alendronate sodium is a potent inhibitor of bone resorption, increases bone mineral density and reduce the risk of fractures in postmenopausal women. The objective of this study was to investigate and develop a new formulation based on Alendronate, to be placed in subcutaneously in the surgical site. Materials and Methods: The pharmaceutical formula was prepared with a dosage of 0.5 mg / kg, he added buffer special, with a final pH of 5.5 means sterile. The control buffers were no active ingredients. The effect of alendronate was evaluated in male Wistar rats (9 weeks of age and weighing 176-214 g, n = 8 per group), which were divided into two groups and one control another problem, the drug is injected in the form subcutaneous times 0, 7, 14 and 30 days. The animals were anesthetized with a solution of Ketamine / xilazina. We performed in the tibia incisions were not filled. All animals were sacrificed by overdose with ether. The parameters studied were watching histopathologic evaluation infiltration of inflammatory cells (ICI), the number and morphology of osteoclasts, gaps reabsorption (surface of the osteoclast) and osteoclast activity. Statistical analysis was performed with the chi-square test. Results: The available data do not reveal any statistically significant difference between the control group and the group with regard to alendronate (ICI), number of osteoclasts and osteoblástica activity. In terms of the morphology of osteoclasts, gaps reabsoción (surface of the osteoclast) and osteoclast activity, the statistics were significant differences between the two groups, favoring the group that used alendronate. Tissue oseo in remodeled, with trabeculae containing osteoblasts with osteocitos viable and important activity osteoblastica and osteoplastica. As for the activity of alkaline phosphatase statistics were glaring differences between the groups. The values were even varying with the day's treatment by deducting the activity of bone with successive treatments. Time 0: 480.00 IU / Time 7:500,00 IU / Time 15:652,00 IU / Time 30:824,00 IU / I Conclusions: These preliminary studies suggest that alendronate placed in parenteral form could to be effective in preventing bone loss in bone diseases of the oral cavity.

Palabras clave : Bisphosphonates; Alendronate; Biopolymers; bone remodeling.