Resumen

CHIRINOS, Marilú et al. Cultivo de anteras en dos clones de yucaAnther culture in two cassava clones. Agronomía Trop. [online]. 2006, vol.56, n.4, pp.633-641. ISSN 0002-192X.

Cassava anther culture is a strategical tool for breeding programs in order to obtain haploid plants, allowing selection of improved clones and detection of very important genes through molecular markers. A complete protocol for cassava haploid plant production has not been developed, therefore we evaluated morphogenic response in two cassava clones: sweet (UCV-2726) and bitter (UCV-2375), for two different sizes (1-1.5 mm and 2-2,5 mm) of floral buds selected in order to ascertain the optimal developmental phase of the microspore. Murashige and Skoog and Chu et al. media were evaluated, supplied with two different auxin concentrations. A factorial 3² completely randomized design and Duncan test were used as statistical methods. For floral bud size of 1-1.5 mm, tetrad and uninucleated microspores were observed. During callus induction, differences were found for bud size with the highest number of calluses formed for 1-1.5 mm in MS medium. The ANAVAR did not show significant differences between clone types and culture media. There were two different mean groups for callus number. Callus tissue differentiated morphogenically into roots; this might be associated with auxins applied to the medium.

Palabras clave : Manihot esculenta; anthers; haploids.