Resumen

MARCANO, Ana Karina; VALDIVIESO, Elizabeth  y  OROPEZA C, Maira. Determination of acid phosphatase activity from embryogenic and non embryogenic sugarcane (saccharum sp.) calluses. Agronomía Trop. [online]. 2006, vol.56, n.4, pp.657-661. ISSN 0002-192X.

Somatic embryogenesis is one of the in vitro propagation systems used more extensively for sugarcane Saccharum sp., culture. During this process changes in the protein expression patterns are observed and they can be determined using isoenzimatic analysis. This approach allows early evaluation of the embryogenic potential before the morphogenetic changes become evident. In this work, the acid phosphatase activity (PAC) from embryogenic (E) and non embryogenic (NE) calluses of sugarcane was studied. Gel electrophoresis was performed using polyacrylamide 10% gels under native conditions, followed by incubation with a-naftil phosphate and ß-naftil phosphate solutions at acid pH. The same activity was determined on enzymatic assays to end point according to Gottlieb and Dwyer (1981), with some modifications. Electrophoretic profiles showed just one band of the same intensity in each extract. Nevertheless, enzymatic assays revealed a higher acid phosphatase activity (1,5 times) in E callus extracts that in the NE. These preliminary results suggest that acid phosphatase activity could be used as a marker for differentiation processes during in vitro culture.

Palabras clave : Sugarcane; Saccharum sp; somatic embryogenesis; acid phosphatase.