Abstract

ROA DELGADO, Sara et al. Molecular characterization of Rubus genotypes by microsatellite markers. Agronomía Trop. [online]. 2014, vol.64, n.1-2, pp.61-72. ISSN 0002-192X.

The andean blackberry (Rubus glaucus Benth), as well as, several wild species in Venezuela are found on hillsides located above 1.500 m. Despite of being an important fruit crop in the country, because of the nutraceutical properties and the great economical value of the activity for the producers, too little is known about its genetic variability and its vulnerability due to the loss of their natural habitats and pest and disease damages. The objective of this work was to characterize the genetic variability of a sample composed by 65 cultivated and wild Rubus genotypes collected in the states of Táchira, Mérida and Aragua. Microsatellite markers were used to characterize the genetic variability. A number of 16 microsatellite primers were evaluated, 6 of which were polymorphic, generating 18 polymorphic fragments with an average of 2.83 alleles per locus. The molecular analysis revealed a great similarity among the R. glaucus genotypes possible due to the use of few especific primers, the vegetative propagation of this crop and that genotypes were collected in nearby geographical regions.

Keywords : Rubus glaucus Benth; blackberries andean; genetic variability; wild species.

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