Resumo

CASTANEDA, Rossmary; RAMIS, Catalina  e  PEREZ, Delis. Association between microsatellite markers and the resistance gene to common bacterial blight in F2:4 families of Phaseolus vulgaris L. Agronomía Trop. [online]. 2014, vol.64, n.3-4, pp.133-141. ISSN 0002-192X.

The incidence of disease is one of the factors limiting the production of black bean (Phaseolus vulgaris L.). The common bacterial blight of bean caused by Xanthomonas phaseoli (Xph) is the most important crop bacterial disease because reduces grain yield by 45% and also affects the quality of the seed. It is necessary to incorporate resistance genes through crossing programs. The object of this study was to verify the association between type microsatellite molecular markers (SSR) and the resistance gene to common bacterial blight in families F_2:4 of black bean. The molecular evaluation was performed using the amplifi cation primers PV-ctt001, PV-atgc001, BM- 140, BM-156 and BM-202, that has previously showed a possible association with resistance. For amplifi cation of SSR, the standardized conditions in the Molecular Genetics Laboratory Agronomy Faculty, Central University of Venezuela were used. Expected segregation of F_2:4 families for each SSR was verifi ed using MapDisto Genetics V 6.3 program. BM-140 behaved as a monomorphic marker and PV-atgc001 as an independent locus, so it was not possible to differentiate between resistant families using these markers. Signifi cant association between SSR: BM-156, BM-202 and PV-ctt001, and resistance controlled by a dominant gene in families F_2:4 was found. Assisted selection to the “b” allele using these SSR will be useful to identify plants carrying the resistance in black bean breeding programs.

Palavras-chave : Xanthomonas phaseoli; genetic markers; plant breeding; black bean.

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