Resumo

ROA, Sara; FERNANDEZ, Hilda; CASTRO, Luís  e  USECHE¹, Nora. Genetic diversity of Rubus species determined by RAPD. Agronomía Trop. [online]. 2014, vol.64, n.3-4, pp.227-235. ISSN 0002-192X.

The andean blackberry (Rubus glaucus Benth) belongs to the family Rosaceae, which includes many cultivated and wild species. In Venezuela R. glaucus, is cultivated in Táchira, Merida, Trujillo, Lara and Aragua states. Promising wild species grow close to R. glaucus, including R. idaeus known as raspberry. Lack of commercial varieties of blackberry, as well as for the preservation of wild species, raises the need for genetic improvement. The objective of this work was the amplifi cation of DNA by RAPD markers in species of the genus Rubus of Táchira and Aragua states, to determine the genetic diversity. DNA was extracted from fresh leaves of R. glaucus, R. idaeus and Rubus spp. 23 RAPD primers were used, and OPA10, OPA18, OPW06, OPB09, OPM10, OPK20 were selected, which produced 44 bands of which 38 were polymorphic. OPW06 was the most informative initiator with a PIC of 0.38. The cluster analysis and principal coordinates separated the species R. Idaeus, other species and also discriminated species from Táchira state with regard to Aragua state. The similarity between genotypes in the same state indicates a probable influence of pollen exchange between wild and cultivated species, as well as the exchange of clonal propagated materials used by the producers. These results may be used in conservation of species and genetic improvement that includes interspecifi c crosses.

Palavras-chave : Rubus glaucus Benth; Rubus idaeus L; blackberry; wild species; ADN; molecular markers.

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