Resumen

ORTIZ DE BERTORELLI, Ligia  y  RAMIREZ, Alejandra. Estudio electroforético de las albúminas y globulinas de cuatro genotipos de Canavalia ensiformis. ALAN [online]. 2000, vol.50, n.1, pp.74-80. ISSN 0004-0622.

Electrophoretic study ofalbumins and globulinsof four genotypes of Canavalia ensiformis. Polyacrylamide gel electrophoresis (PAGE) and PAGE-SDS were used to study seed albumins and globulins of Canavalia ensiformis. In PAGE the concentration of acrylamide used in the upper gel was 4.0% with a pH of 6.7 and in the lower gel 7.5% and 10% of acrylamide were used with a pH of 8.9. In P AGE-SDS the concentration of acrylamide was 4.4% with a pH of 6.8 in the upper gel and 7 .5% and 12.6% with a pH of 8.8 in the lower gel. The material used were the Venezuelan genotypes Tovar. Yaracuy, Original and U-02. The albumins and globulins were extracted with a 0.5 M NaCI solution and then separated by dialysis against water and lyophilized. These protein fractions represented 84.85% of the total amount of protein in seeds. The albumins were separated in PAGE with 7.5% acrylamide into five fractions and globulins into six, with similar electrophoretic pattems between genotypes. In a similar manner, the patterns obtained in PAGE with 10% acrylamide were the same for all genotypes, showing five bands for albumins and three bands for globulins. With P AGE-SDS containing 7.5% of acrylamide, albumins were separated into as many as eight components, and globulins into as many as seven bands with mobilities between 0.2981 and 0.9932, with different pattems for each genotype. Also the pattems P AGE-SDS at 12.6% of acrylamide were different for the genotypes, separating proteins into a greater number of bands. The albumins showed as many as twcntyone bands with mobilities between 0.2603 and 0.7398, and globulins as many as sixteen bands with mobilities between 0.2454and 0.7390. The P AGE pattems of the genotypes analyzed did not show differences between them. However, with PAGE-SDS different electrophoretic pattems were obtained which varied in the number and intensity of the bands, making it possible to distinguish the genotypes studied. The molecular weight of the albumins varied between 76.000 and 12,000 daltons and of the globulins between 80,000 and 12,000 daltons.

Palabras clave : Canavalia; albumins; globulins; electrophoresis.