Abstract

SORENTINO, Rebeca et al. Prostaglandina tisular y niveles séricos del factor de necrosis tumoral alfa (TNF-α) en un modelo experimental de rectocolitis aguda en ratas. Gen [online]. 2007, vol.61, n.2, pp.132-135. ISSN 0016-3503.

Introduction: In murine models of bowel inflammation, in a similar way that occurs in humans, activated macrophages produce a powerful mixture of inflammatory ciytokines that influence the tumor necrosis factor alpha (TNF-α). The TNF-α, increases the inflammatory process and tissue destruction, producing the symptoms of the disease. Non steroidal antiinflamatory drugs (NSAID's) inhibit the production of prostaglandins and have been recognized as inhibitors of the TNF-α production. In this study we correlate prostaglandin inhibition using Celecoxib with TNF-α levels in rats with bowel inflammation comparing results with non treated rats. Methods: The TNF-α levels were determined in rats with induced colitis with acetic acid. The control group was represented by 4 rats administered with distilled water, a group of 7 rats without treatment and three groups of 7 medicated rats each one with different Celecoxib concentrations. At the third day the rats were sacrificed. All rats had blood samples taken before inducing colitis, and after being sacrificed. Histological samples were taken from the colon for pathological study. TNF-α levels were determined by ELISA. Results: Edema, erosion and ulceration were less in the treated rats that in the non treated. TNF-α levels in the treated group were significantly lower when compared with the non treated group. Conclusions: Rats treated with COX-2 inhibitor have lower TNF-α levels and minor injuries to the colonic mucosa, for these reasons it could develop into an important therapeutic weapon for inflammatory bowel disease.

Keywords : Celecoxib; Factor of Tumorlike Necrosis Alpha; Prostaglandins.