Resumen

SALAZAR RIVERO, Esmely. Detección del virus de papiloma humano en pacientes con lesiones intraepiteliales escamosas de cuello uterino. Rev Obstet Ginecol Venez [online]. 2007, vol.67, n.1, pp.47-54. ISSN 0048-7732.

SUMMARY Objective: To detect and typify the human papilloma virus, in patients with escamous intraepithelial lesions in uterine cervix by polymerase chain reaction. Method: Sample for polymerase chain reaction was taken to 100 patients, and cervical cytology, colposcopy and biopsy were performed. Exclusion criteria: patients with leucorrhea, genital bleeding and human papilloma virus infection treated local o systemically. Setting: Consulta de Ginecologia del Hospital Universitario “Antonio P. de Alcalá”, Cumana, Estado Sucre. Results: 98 % of the samples presented viral genome for polymerase chain reaction and 2 % were negative. The 52.38 % of cases with NIC I presented human papilloma virus type 16, 23.81 % type 18 and 23.81 % type 31. In the patients with NIC II; 23.81 % were associated to human papilloma virus type 11 and 76.19 % to type 31. A 37.5 % of cases with NIC III were carriers of human papilloma virus type 31. To patients with invasive carcinoma, human papilloma virus type 16, of high oncogenic risk, was detected. In 99.98 % of patients a punteado was observed as the more frequent abnormal colposcopic finding. Comparing the molecular hybridization studies with cytology and cervical biopsy, cytology has a sensitivity of 51 %, and a specificity of 100 %. Cervical biopsy, a sensitivity of 62 % and a specificity of 100 %. Conclusions: Polymerase chain reaction constitute a methodology of high sensitivity for the diagnostic of human papilloma virus infection, and permit the identification of adequate lesions for the conservative treatment and detection of cancer precursors, for a more sensible and efficacious cervicouterine pathology.

Palabras clave : Human papilloma virus; Polymerase chain reaction; Escamous intraepitelial lesions.