Resumen

QUINTERO VEGA, Militza et al. Detección y tipificación de virus del papiloma humano (VPH) mediante PCR- RFLP. Rev Obstet Ginecol Venez [online]. 2008, vol.68, n.1, pp.25-31. ISSN 0048-7732.

Objective: To standardize a PCR-RFLP technique for detection and typification of HPV in women with clinical diagnostic of HPV infection. Method: Descriptive and transversal study with the assessment of 189 samples of the genital area of women that required DNA extraction, diagnostic and typification for PCR-RFLP molecular technique. Setting: Facultad de Ciencias, Laboratorio de Biologia y Medicina Experimental LABIOMEX, Universidad de Los Andes. Merida, Estado Merida, Venezuela. Results: The PCR-RFLP was able to detect and identify the virus. The 16.8 % of the samples presented HPV of high types 16, 31, 33, 35, 56, 59 and 68; the 6.8 % presented HPV of intermediate risk types 51, 53, 58, 61 and 83; and 18 % the low risk types 6, 32, 53, 54, and 81. Conclusions: The standardized PCR-RFLP technique was appropriate for the diagnostic and typification of HPV in samples from the genital area. The 51.9 % of samples were positive to HPV, been the more common (20 %) the HPV high risk subtype 16, and the low risk HPV 6 the more frequent (23.8%).

Palabras clave : Human Papillomavirus; Cervical infection; PCR-RFLP; Cervical cancer.