Abstract

DE GUGLIELMO, Zoraya et al. Evaluación, mediante RCP, de la infección por el virus de papiloma humano en muestras de pacientes con diagnóstico clínico o citológico. Rev Obstet Ginecol Venez [online]. 2008, vol.68, n.4, pp.240-247. ISSN 0048-7732.

Objectives: The aim of this study was the HPV detection and typification by PCR with MY09 and MY11 primers and a Multiplex-PCR assay, in patients samples with cytological diagnostic of koilocytosis and low grade squamous intrapithelial lesion, or clinical diagnostic of condylomatosis and papillomatosis. Methods: Seventy cytological samples of the genital area were assessed for DNA extraction and viral detection and typification by molecular techniques (PCR and Multiplex PCR). Setting: Laboratorio de Genética Molecular-Instituto de Oncología y Hematología, Results: From 70 evaluated samples, 31 were positive for HPV detection, with 29 samples corresponding to low risk HPV (types 6 and/or 11), one corresponding to high and low risk HPV mixture infection (types 6 and 33) and one corresponding to high risk HPV coinfection (types 18 and 33). Conclusions: These results reflect the PCR utility to the HPV detection and typification, and evidence a well known relation between low risk HPV and moderated displasias, as well as the possible overstimate of HPV infection in the reference diagnostic.

Keywords : HPV; SIL; PCR; Cytology.