Resumen

CRUZ GOMEZ, Jhon Fredy et al. Distribución del polimorfismo del codón 72 del gen p53 en lesiones de cuello uterino. Rev Obstet Ginecol Venez [online]. 2010, vol.70, n.1, pp.31-36. ISSN 0048-7732.

Objective: To determine the distribution of the polymorphism of the codon 72 of the gene p53 in patients that present cervical lesions associated to infection by VPH. Method: Descriptive and transversal study through assessment of 118 samples of the genital feminine area were processed, of 59 healthy (control) women and 59 with cervical lesions NICI-NICII-NICIII and Ca in situ (cases), for the extraction and purification of the DNA. The exon 4 of the gene p53 was amplified, for the genotipification of the codon 72 by means of technical PCR-SSCP. Setting: Facultad de Ciencias, Laboratorio de Biologia y Medicina Experimental LABIOMEX, Universidad de Los Andes. Merida, Estado Merida, Venezuela. Results: PCR-SSCP allowed determining the frequency of the homozygotes genotypes arginine (Arg/Arg), proline (Pro/Pro) and heterozygotes proline /arginine (Pro/Arg). For the cases the genotype Arg / Arg had a frequency of 32.20 % and for the controls of 50.85 %. The genotype Pro/Pro was in 5.09 % of the cases and 11.86 % for the controls. The genotype Pro/Arg had a distribution of 62.71 % for the cases and 37.29 % for the controls. Conclusion: In this investigation there was not a statistically significant relationship among the presence of the genotype Arg/Arg and the development of cervical lesions.

Palabras clave : HPV; P53; Codon 72; PCR-SSCP.