Resumen

CARRILLO ALESSANDRO, Coralia et al. Detección del virus papiloma humano: influencia del tipo de muestra y la severidad de la lesión intraepitelial cervical. Rev Obstet Ginecol Venez [online]. 2010, vol.70, n.4, pp.240-248. ISSN 0048-7732.

Objective: Identify the influence of the kind of sample and the severity of the injury, to detect the desoxyribonucleic acid of the human papilloma virus using polymerase chain reaction. Method: Between October 2008 and January 2009, 68 patients with histological diagnosis of intraepithelial lesion were selected, making detection of human papilloma virus desoxyribonucleic acid in polymerase chain reaction, in both, histological and cytological cervical sample. Analysed the results grouped by type specimen (cytological and/or histological) and type of lesion (high or low grade). Setting: Service of Gynecology of the Maternidad "Concepcion Palacios", Results: The sensitivity of the polymerase chain reaction for the detection of human papilloma virus desoxyribonucleic acid in histological sample was 92.98 %, higher than the cytological 72.06 % (P < 0.05). The severity of the lesions didn’t afect the detection capability of polymerase chain reaction. (P>0.05). The sensitivity of the extended in low grade lesions was 65 % and the biopsy was 97.2 %; while in high-grade lesions was 82.14 % (extended) and 85.71 % (biopsies). Conclusions: The severity of intraepithelial lesion (high or low grade) does not affect the effectiveness to detect human papilloma virus desoxyribonucleic acid. Detection was more effective in histological samples than in cytological.

Palabras clave : Human papilomavirus; High-grade intraepithelial lesions; Low grade intraepithelial lesions; Polymerase chain reaction.