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Revista de Obstetricia y Ginecología de Venezuela
versión impresa ISSN 0048-7732
Resumen
HERNANDEZ, Danmarys et al. Diseño de un sistema de PCR para la detección del virus del papiloma humano mediante el uso de oligonucleótidos degenerados de la región E6. Rev Obstet Ginecol Venez [online]. 2012, vol.72, n.4, pp.249-254. ISSN 0048-7732.
Objective: Design and standardize a PCR system to detect a broad spectrum of HPV types in a single reaction using target the E6 region of the viral genome. Methods: Using E6 gene sequences of different HPV types and NCBI tools Mult Alin Oligo Analyzer version 1.1.2 5.4.1. Degenerate oligonucleotides were designed that allowed amplification of a fragment of approximately 214 bp. Selected samples include: 25 DNA samples, each positive for a single HPV type typified by PCR-RFLP system MY11/MY09 L1 gene. Results: We obtained approximately 214 bp amplified from 25 different HPV types were detected in samples amplified from E6 negative MY09/MY11 system. Conclusions: It was determined that the design of degenerate oligonucleotides was highly efficient for amplification of at least 25 different HPV types which would facilitate detection in clinical samples.
Palabras clave : HPV; E6 gene; PCR; MY09/MY11.