Resumen

CRUZ GOMEZ, Jhon Fredy et al. Estudio de variantes intra-tipo del virus del papiloma humano tipo16, por análisis nucleotídico de la región MY09-MY11. Rev Obstet Ginecol Venez [online]. 2013, vol.73, n.3, pp.187-194. ISSN 0048-7732.

Objective: Identify variants of HPV-16 intratiping by analyzing the region MY09 / MY11 L1 gene, en el Laboratory of Experimental Biology and Medicine LABIOMEX, Universidad de Los Andes. Merida. Methods: Cross sectional study in which 45 samples were processed in the genital area, 38 female and 7 male, with infection by HPV16. Polymorphic analysis was performed on single stranded DNA (SSCP) in order to discover intratipo polymorphic differences were analyzed different polymorphisms found by automatic sequencing and phylogenetic relationships were established and functional variants found. Results: Intratipo able to detect three variants of HPV16 in the study population in the region MY09 / MY11 L1 gene. Variants correspond to nucleotide substitutions synonymous. It was determined that 3 of HPV16 variants detected belong to the European class. Conclusion: The analysis of the polymorphic region MY09/ MY11 allowed determining the presence of intratipe variability in the HPV16 L1 gene. All variations were kind of mutation, there were no insertions or deletions. We identified the sequences of HPV-16 reference clone, which is important for the development of diagnostic systems, characterization and vaccines.

Palabras clave : Human papilloma virus; Cancer; Intratipe variants; PCR; RFLP; SSCP; Phylogeny.

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