Abstract

LOZANO-HERNANDEZ, Ricardo; SALDIVIA, Manuel  and  VILLAVICENCIO, Antonio. Concentración espermática mínima requerida para inseminación intrauterina mediante capacitación por migración ascendente. Rev Obstet Ginecol Venez [online]. 2014, vol.74, n.3, pp.177-183. ISSN 0048-7732.

Objective: To determine what is the minimum necessary amount of motile sperm required for intrauterine insemination and to evaluate the Kruger strict morphology test and sperm motility before and after training sperm by swim up. Methods: Prospective study of 35 semen samples from infertile men, aliquots of 1 mL of fresh semen was washed, centrifuged and over the pellet was placed a layer of capacitation medium to achieve an upward migration. Results: The values of motility and normal sperm forms were observed in the samples significantly increased after the training. It was possible to recover ≥2 x 106 motile sperm even in seemingly inappropriate samples with hypospermia or severe oligozoospermia, but these contained in the total ejaculate at least 5 million of motile spermatozoa that allowed a high percentage of retrieval. Conclusions: The possibility of obtaining high recoveries of motile sperm in the total ejaculate allows IUI as assisted reproduction technique in oligozoospermic patients before choosing IVF or cytoplasmic sperm injection when the male factor is cause of infertility.

Keywords : Intrauterine insemination; Sperm capacitation; Strict morphology of Kruger.

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