Abstract

AGUIAR, Henry et al. Asociación entre presencia del virus del papiloma humano y hallazgos anatomo-patológicos. Rev Obstet Ginecol Venez [online]. 2015, vol.75, n.3, pp.164-171. ISSN 0048-7732.

Objective: To determine by Polymerase Chain Reaction the presence of the human papillomavirus in female patients attending a screening for lesions of uterine neck in the outpatient network of Francisco Linares Alcantara Municipality and to relate the results to anatomopathological findings. Methods: After obtaining informed consent from patients, samples of vaginal swabs were taken from 301 women that were used for cytology and colposcopy studies. Also, DNAs were isolated and they were used for Polymerase Chain Reaction test coupled to restriction enzyme digestion. Statistical tests were performed to analyze the relationship between the human papillomavirus positivity and the variables: stratus age, the onset of sexual activity, number of sexual couple, cytology and colposcopy findings. Results: Out of 43 human papillomavirus positive samples, 17 (39.53 %) were genotype 16, 3 (6.98 %) genotype 18 and 1 (12.33.%) genotype 33; 14 (32.56 %) samples showed coinfection and 8 (18.60 %), samples were not digested with the restriction enzymes used. The relationship between the presence of human papillomavirus and the others studied variables (stratus age, the onset of sexual activity, number of sexual couple and cytological results) was statistically significant. Conclusions: The presence of human papillomavirus genotypes, of so-called high risk in the 48.84 % of the women with a positive HPV test is a particular concern, because they are associated with the development of cervical cancer.

Keywords : Genotyping; Human papillomavirus; RFLP; Polymerase Chain Reaction.

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