Resumen

REBOLLEDO PULIDO, Vilma; COLOMBO SILVESTRE, Carla  y  REBOLLEDO PULIDO, Luis. Expresión de citoqueratinas en el cáncer de mama y subtipos tumorales por inmunohistoquímica. Rev Obstet Ginecol Venez [online]. 2016, vol.76, n.2, pp.93-101. ISSN 0048-7732.

Objective: To characterize breast cancer molecular subtypes by immunohistochemical expression of Ck5 / 6, Ck7, and Ck18 Ck8 markers associated with certain classical and analyze the clinical evolution of different subtypes. Methods: Sample was intentional and according to defined criteria including paraffin blocks 149 cases with infiltrating ductal carcinoma of the breast was selected. Previous results of molecular markers by immunohistochemistry that included ER, PR, Her-2, p53, Bcl-2 and Ki-67 immunostaining of the first three was analyzed and tumor subtype was established. Paraffin blocks 5 tissue microarrays were constructed and immunohistochemical expression of Ck5/6, Ck7, Ck8, Ck18 according to tumor subtype was evaluated. Results: The mean age of patients was 51.32 years. The A and B luminal tumors expressed CK18 Ck8 and over 80 % of cases. The Her-2 + tumors expressed in over 80 % of Ck7 and Ck18 cases, the Bcl2 protein and p53 marked in less than 25 % and 39 % thereof. TN tumors expressed Ck5/6, Ck7, and Ck18 Ck8 variable form. p53 and Bcl2 protein immunostained in variable percentage of cases and Ki-67 in almost all. They showed most tumor recurrence Her-2 + and TN, overall survival was lower in patients with these subtypes. Conclusion: The analysis of molecular biomarkers in this study provides a better definition of each tumor.

Palabras clave : Breast carcinoma; Cytokeratin; Molecular subtype.

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