Resumen

ROJAS, Lisbeth et al. Estandarización de un sistema PCR multiplex para la determinación molecular de infertilidad masculina idiopática. Rev Obstet Ginecol Venez [online]. 2016, vol.76, n.3, pp.277-283. ISSN 0048-7732.

Objective: To design and optimize individual systems and multiplex polymerase chain reaction for detection of microdeletions of male infertility associated genes on the Y chromosome. Methods: multiplex polymerase chain reaction systems were standardized using oligonucleotides STS (Sequence Target Site) specific to male infertility associated with prior standardization of each pair of oligos in individual reactions. Results: It was possible to standardize 7 individual systems and two multiplex systems high sensitivity and specificity that may indicate the presence or absence of a gene, in this case, are used to indicate the mutation microdeletion of a specific fragment Yq leading to the inactivation of a gene. Conclusions: standardization could make two multiplex systems for the analysis of microdeletions of the Y chromosome associated with male infertility as a molecular tool for rapid and accurate diagnosis of this disease. The amplified RBM1 marker could not be included in either standard multiplex, despite the studies of other markers of male infertility is suggested to be included in new in the standardization of new multiplexes.

Palabras clave : Male Infertility; Microdeletions; Y Chromosome; PCR; STS.

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