Resumen

CASTELLANO GONZA¡LEZ, Maribel; PEROZO MENA, Armindo  y  GONZA¡LEZ, Ana Isabel. Evaluation of Susceptibility to Glycopeptides in Oxacillin Resistant Staphylococcus aureus Strains . Kasmera [online]. 2012, vol.40, n.1, pp.7-15. ISSN 0075-5222.

The decrease in susceptibility to glycopeptides by oxacillin resistant Staphylococcus aureus (ORSA) has become a current problem. Objective: To evaluate susceptibility to glycopeptides in ORSA strains isolated in the University Hospital, Maracaibo, during the first quarter of 2010. Methods: Oxacillin resistance of the ORSA strains was verified by diffusion on agar (oxacillin 1 µg and cefoxitin 30 µg, OXOID^®), a screening test in Müeller Hinton agar with 6 µg/mL of vancomycin (Sigma^®), and E-test® for minimum inhibitory concentration (MIC) for oxacillin, detection of PBP2a (Latex, OXOID^®) and the mecA gene by polymerase chain reaction (PCR). In addition, susceptibility to glycopeptides was determined by disk diffusion (teicoplanin, 30 µg OXOID^®), MIC by E-test^®, screening test in Müeller-Hinton agar with teicoplanin (5 µg/ml) or in brain heart infusion agar with vancomycin (6 µg/mL), glycopeptide hetero-resistance detection by GDR E-test^® and detection of the vanA gene using PCR. Results: No ORSA strain was isolated with intermediate resistance (VISA or hVISA), nor with high-level resistance to glycopeptides. Conclusions: Locally, there is no incidence of ORSA strains resistant to glycopeptides; therefore, these antibiotics continue to be useful for treating infections produced by this important pathogen.

Palabras clave : ORSA; glycopeptides; resistance.

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