Abstract

DAVILA-PEREZ, Jesús Enrique  and  MARTIN-LANDROVE, Rafael. Delay in fructose-mediated renaturalization process for irradiated triple-helix gelatin chains in aqueous medium. Rev. LatinAm. Metal. Mater. [online]. 2016, vol.36, n.2, pp.131-143. ISSN 0255-6952.

The triple-helix denaturalization in gelatin as a result of its interaction with ionizing radiation and evolution of renaturalization process were studied in a hydrated mixture of 250 Bloom type B gelatin, fructose and tridistilled water (gelatin + fructose), which was irradiated with X-rays produced by a clinical linear accelerator with an operating potential of 6 MV in order to deliver a dose between 30 and 300 Gy. Irradiated and non-irradiated samples were analyzed with the use of mass spectroscopy and magnetic resonance imaging (MRI) with diffusion techniques. The mass spectroscopy results indicate that no formation of new compounds took place for delivered doses. With diffusion techniques in a magnetic resonance 1.5 T scanner a follow-up of macroscopic changes in gelatin + fructose mixture could be done as they correspond to a triple-helix denaturalization followed by its incomplete renaturalization, which is an indication that changes in the material porous structure at microscopic level happened with a renaturalization half life time of 12.46 ± 0,15 h.

Keywords : Gelatin; Fructose; Renaturalization; Radiation; Mass Spectroscopy; Magnetic Resonance Imaging.

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