Resumo

GONZALEZ-MUJICA, Freddy; PRADERE, José D  e  GONZALEZ, Julio C. Renal Glycosuria Induced by Acute Thioacetamide Administration to Rats. Rev. Fac. Cienc. Vet. [online]. 2007, vol.48, n.2, pp.111-119. ISSN 0258-6576.

Thioacetamide (TAA) administration to rats (75 mg/kg body weight by subcutaneous injection) produced a statistically significant increase in the urinary excretion of glucose, Na+ and phosphate. In addition, there was a decrease in the arterial pressure and in the glomerular filtration rate, without changes in the urinary excretion of K+, H+, amino acids or in the glycaemia. Decreases of 60% and 75% were observed in the Tmax and Tmin for glucose, respectively. TAA intoxication reduced in 16% the overshoot, by almost 63% the Vmax and by nearly 82% the Km of the brush border membrane vesicles (BBMV) [14C]-glucose uptake. It also decreased about 52% the Kd and by almost 59% the number of binding sites of BBMV [3H]-phlorizin. These results strongly suggest that TAA reduces the amount of Na+/glucose co-transporter (SGLT1) present in the apical membrane of the proximal renal tubule. An increase in the BBMV fluidity is suggested by the decrease in the BBMV maltase and alkaline phosphatase transition temperature and by the increase in the unsaturated fatty acids content of BBMV phospholipids. These results may explain the increase in SGLT1 affinity for glucose evidenced by the decrease in the Kmof the BBMV [14C]-glucose uptake and the Kd of the BBMV [3H]-phlorizin binding caused by TAA intoxication

Palavras-chave : Rat; thioacetamide; carcinogens; glycosuria; SGLT1.

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