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Revista de la Facultad de Ciencias Veterinarias
versión impresa ISSN 0258-6576
Resumen
FERNANDEZ, Adriana et al. Effect of the Reproductive Status of Crossbred Zebu Cows on In Vitro Embryo Production. Rev. Fac. Cienc. Vet. [online]. 2012, vol.53, n.2, pp.89-96. ISSN 0258-6576.
In order to improve the oocyte selection for in vitro embryo production, the present work evaluated the effect of the reproductive status of postmortem Crossbred Zebu cows (pregnant [P] versus non-pregnant [NP]) on the number of recovered oocytes, the cleavage (number of cleaved embryos/number of recovered oocytes) and the blastocyst production (number of blastocyst/number of cleaved embryos) rates. Cumulus oocytes complexes (CCOs) were selected, counted and separated depending on the donor status: P cows with or without a corpus luteum (CL) and NP cows with (CL) or without (NoCL) a CL. Data for number of recovered oocytes was analyzed using the GENMOD procedure assuming a Poisson distribution, whereas data for cleavage and blastocyst production rates were analyzed using a logistic regression assuming a binomial distribution. The statistical model for all the analyses included the effects of the reproductive status (P vs. NP), presence or not of a CL (CL vs. NoCL) and the interaction of reproductive status x CL. A total of 4658 CCOs obtained from 437 ovaries was evaluated. The number of CCOs collected per ovary from P cows (12.5±2.8) was significantly higher (p<0.03) than from NP cows (9.4±2.5). On the other hand, the cleavage rate was significantly higher (p<0.006) in NoP cows (42.3+0.01%) than in P cows (34.5+0.02). Nevertheless, the interaction between reproductive status and CL affected (p<0.006) the cleavage rate: P-CL: 31.1+0.02% (76/244); P-NoCL: 38.0+0.03% (98/258); NoP-CL: 46.7+0.03% (312/738) and NoP-NoCL: 38.0+0.03% (143/376). There were not differences in blastocyst production rate from cleaved embryos for P cows (10.7+0.02%; 17/160) compared with NoP cows (10.0+0.02%; 30/312). These results show that the reproductive status of a cow should be taken into account for oocyte selection, in order to improve embryo production.
Palabras clave : Reproductive status; cattle; zebu; in vitro embryo production; oocytes.