Resumen

SAAVEDRA, Diógenes; OJEDA, Álvaro; LUZON, Odalis  y  MAZZANI, Claudio. Effect of Mycotoxin Binder on in Vitro Gas Production and Ruminal Degradation of Maize Meal with Aflatoxin B1. Rev. Fac. Cienc. Vet. [online]. 2014, vol.55, n.2, pp.96-103. ISSN 0258-6576.

To evaluate the effect of a commercial mycotoxin binder (MB) based on glucomannans, on the in vitro gas production and ruminal degradation, maize (Zea mayz L.) grain meal was incubated in ruminal liquor with aflatoxin B1 (AFB1) concentrations of 4, 8 y 12, respectively, and proportions of AFB1: MB of 1:75000 (MB-75), 1:150000 (MB-150), and 1:225000 (MB-225). Additionally, a treatment without MB was considered (MB-0). Gas production was measured at 3, 6, 9, 12, 16, and 24 h, and MB activity after 3 h of incubation. Data was analyzed using a completely randomized design with a 3x4 factorial arrangement. The results show that gas production was negatively affected by AFB1 concentration (P<0.05), with the higher impact at 9 h, when 12 ppb [5.15 mL/g of dry matter (DM)] generated a reduction, when compared to 4 and 8 ppb (6.57 and 6.01 mL/g of DM, respectively). Regardless of the level used and after 24 h of incubation, the use of MB increased (P<0.05) gas production in 25.5%, compared to SM-0 (68.5 vs. 56.6 mL/g of DM, respectively). In all treatments, MB-75 showed the highest gas production without improvements, due to further increases in the ratio MB: AFB1. The AFB1 concentration negatively affected (P <0.05) degradation of DM of maize meal, with a reduction of 39.8%, after 12 h of incubation. The binding capacity was 86.0 ± 3.34%, without differences (P<0.05) due to AFB1 concentration or AFB1: MB relation. These results demonstrate that the assessed biotechnological additive reduces the negative impact of AFB1 on the in vitro ruminal gas production and apparent DM degradation.

Palabras clave : Aflatoxins; gases; in vitro; Aspergillus flavus; corn flour; metabolic detoxification; rations.

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