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Revista de la Facultad de Ciencias Veterinarias
versión impresa ISSN 0258-6576
Resumen
TIQUE, Vaneza et al. Brucella sp. seroprevalence in equids of Córdoba, Colombia. Rev. Fac. Cienc. Vet. [online]. 2016, vol.57, n.2, pp.92-100. ISSN 0258-6576.
Brucellosis is considered one of the most important zoonotic diseases in the world because of its implications for public health and its impact on livestock production. In the equine species, the presentation of this disease is important, because these animals are potential hosts and contribute to the introduction of the disease in unaffected areas, as well as to the maintenance of this disease in endemic zones. In the Department of Córdoba, Colombia, there have been no recent studies that establish the prevalence and epidemiological characteristics of the disease in equids. The purpose of the present study was to estimate the prevalence of Brucella sp. in equids of the department of Córdoba. A cross-sectional descriptive study was carried out involving 506 equids from 14 municipalities of the department. Three species from 46 farms were studied: horses (n=257), asses (n=130) and mules (n=119). The techniques used for the serological diagnosis were: rapid agglutination, which was performed in 506 equids, using the Bengal Rose (RB) method; and the confirmatory method was performed on 23 equids, using the competitive ELISA tecnique (C-ELISA). Conventional PCR was performed by amplifying the IS711 region of Brucella sp. from exudate samples (n=2) and EDTA blood (n=4). The results of the study showed that the seroprevalence of brucellosis in equids was 4.5% (23/506) using the RB methodology, with the following distribution by species: 20 horses, 2 asses and 1 mule. With the C-ELISA technique, the seroprevalence was 4.3% (1/23), whereas by PCR no bacterial DNA was detected in the six samples analyzed. The positivity in farms was 28.2% (13/46). In conclusion, the seroprevalence figures obtained in the present study demonstrate the importance of epidemiological surveillance in the different species susceptible to brucellosis.
Palabras clave : Brucellosis; Equid; Rose Bengal; PCR.