Resumen

MOLINOS DA SILVA, Christiane et al. Efecto del potencial osmótico y contenido de Ca en el medio de cultivo sobre la distribución de Ca²⁺ Y K⁺, producción de biomasa y necrosis apical de VID "R110". INCI [online]. 2004, vol.29, n.7, pp.384-388. ISSN 0378-1844.

The osmotic potential, ionic balance and the nutrient ratios of culture medium are determinant for in vitro propagated plants growth; however, in most investigations, these factors are not considered. The effect of different osmotic potentials (-0.106, -0.113, -0.120, -0.128, -0.143 and -0.157MPa) and Ca²⁺ contents of culture medium salts on the distribution of Ca²⁺ and K⁺, and on dry biomass of explants of grapevine were evaluated. The Ca²⁺ and K⁺ concentrations were measured with a low vacuum scanning electron microscope (MEB-LV). Ca²⁺ and K⁺ translocation from stems to leaves was favored by the higher osmotic potential of culture medium (-0.106MPa), whereas the larger contents of these elements at the base of the stems were found at the most negative osmotic potential (-0.157MPa). The greater dry biomass weight (57mg) and the smaller percentage of explants with Ca²⁺ deficiency symptoms (20%) were obtained at -0.106MPa, the lower osmotic potential, and 1.5mM Ca²⁺ in the culture medium.

Palabras clave : Portainjerto; Relación Ca²⁺/K⁺; Vitis berlandieri x V. rupestris R.