Abstract

FERNANDEZ-DA SILVA, Rafael; HERMOSO-GALLARDO, Luis  and  MENENDEZ-YUFFA, Andrea. Primary and secondary somatic enbryogenesis in leaf sections and cell suspensins of coffea arabica cv. catimor. INCI [online]. 2005, vol.30, n.11, pp.694-698. ISSN 0378-1844.

The main purpose of this research was to characterize and optimize the development of secondary somatic embryos in coffee (Coffea arabica cv. Catimor). In addition, as the primary somatic embryos formation is the previous stage to the differentiation of secondary somatic embryos, three methods for its induction were compared: 1) solid media in two stages, first in a medium with 4.5µM of 2,4-dichlorophenoxiacetic acid (2,4-D) and 35µM of benzyladenin (BA) and then in a medium with 4.3µM naphtaleneacetic acid, 2) solid media with 4.4-53.3µM BA in one stage, and 3) suspension cultures. In the latter treatment the callus was formed in two stages of solid media, one containing 9.3µM of kinetin and 2.3µM of 2,4-D, and a second one with 22.2µM of BA, and a third liquid medium with 35µM of BA. All the treatments induced primary and secondary somatic embryogenesis. The highest yield of somatic embryos was obtained in suspension cultures. The secondary somatic embryos differentiated directly from epidermal and subepidermal cells of the hypocotilar zone of the primary somatic embryos.

Keywords : Benzyladenine; Direct Somatic Embryogenesis; Histology; Indirect Somatic Embryogenesis; Leaf Explants; Primary Embryos; Secondary Embryos.