Abstract

GOMEZ, Cristian; MATILDE URIBE, Darcy Ríos  and  SANCHEZ-OLATE, Manuel. EMBRYOGENIC CALLUS INDUCTION IN Eucalyptus globulus Labill. INCI [online]. 2006, vol.31, n.10, pp.734-738. ISSN 0378-1844.

Embryogenic callus induction starting from three explants coming from mature seeds (mature zygotic embryos, hypocotils and cotyledons) was studied. The results indicated that the medium I3 (B5 + ANA 0.5mg·l^-1 + BAP 1.0mg·l^-1) produced the largest percentages of callus formation in all the explants. Micromorphology revealed the presence of embryogenic cells in all explants, while globular states in initial phases were found in hypocotils and cotyledons. Later, in the differentiation phase, a maximum of 25 globular states by callus were obtained in a B5 medium with maltose (2% p/v) added, when using mature zygotic embryos. The histological study showed internal differentiation of globular states, characterized by a concentric formation of vascular bundles, parenchymatous cells towards the periphery and, finally, a protodermis formed by isodiametrical cells.

Keywords : Embriogénesis Somática; Embriones Somáticos; Estados Globulares; Eucalyptus globulus.