Abstract

ROYERO, Marilyn; VARGAS, Teresa Edith  and  OROPEZA, Maira. Micropropagation and organogenesis of Dioscorea alata (Ñame). INCI [online]. 2007, vol.32, n.4, pp.247-252. ISSN 0378-1844.

To establish an efficient in vitro regeneration system for D. alata plants, yam tubers were potted in a mixture of soil and organic humus in greenhouse conditions. For micropropagation 1cm long stem sections with 1-2 lateral buds were obtained from these plants. For organogenesis, micropropagated plants were used as sources of explants (microcuttings). Explants were cultured on Murashige and Skoog (1962) media (MS) supplemented with different hormonal combinations. For micropropagation, MS and 1/5 MS were used as control media. Three more media with hormones were also used for micropropagation. After 45 days, 4.9 buds per explant were obtained on MS supplemented with 0.5mg·l^-1 BA. Mass multiplication was achieved in MS supplemented with 2mg·l^-1 BA. After 90 days an average of 5.75 plants per explant was obtained. MS supplemented with 1mg.l^-1 BA + 0.5mg·l^-1 ANA was used for the establishment of organogenesis. After 105 days, an average of 25.15 buds per explant was obtained (direct organogenesis). Callus tissue production was observed on 10% of microcutting explants cultured on the same medium with an average of 5.3 buds per 1cm² callus fragment (indirect organogenesis). Micropropagated D. alata plants were obtained after 4.5 months of culture; while plants originated through direct organogenesis took 6 months to reach maturity; however, they produce a higher number of buds per explant. D. alata plants regenerated through micropropagation and organogenesis processes were potted with soil and river sand (1:1) and 70.7% plant acclimatization was obtained.

Keywords : Dioscorea alata ; Micropropagación ; Organogénesis .