Abstract

MARYS, Edgloris  and  CARBALLO, Octavio. Development of a diagnostic tool for the rice "hoja blanca" virus in Venezuela. INCI [online]. 2007, vol.32, n.4, pp.262-265. ISSN 0378-1844.

The development of a DAC-ELISA assay for the diagnosis of Rice hoja blanca virus (RHBV) in Venezuela is reported. The virus was isolated and partially characterized from infected rice samples collected in the states of Portuguesa and Guárico. Highly specific immunoglobulin (IgGs) to virus particles reacted in 1:1000 dilution, when tested against viral particles by immunoassay. According to serological data, the incidence of RHBV in the main rice growing states was low (11%), possibly due to the use of resistant rice lines by local farmers and/or to the low incidence of the virus vector, the insect Sogatodes orizicola. The diagnostic will allow phytosanitary certification of RHBV free material.

Keywords : Arroz ; Diagnóstico ; Hoja Blanca ; Incidencia ; Venezuela ; Virus .