Resumen

MARQUEZ-ARAQUE, Alis Teresa et al. Fibrolytic activity of enzymes produced by Trametes sp. EUM1, Pleurotus ostreatus IE8 and Aspergillus niger AD96.4 in solid fermentation. INCI [online]. 2007, vol.32, n.11, pp.780-785. ISSN 0378-1844.

The enzymatic activity (UI·g^-1·dry matter) and specific activity (UI·mg^-1 protein) of xylanases, celullases and laccases from the fungi Trametes sp. EUM1, Pleurotus ostreatus IE8 and Aspergillus niger AD96.4 was studied at 14 and 19 days of solid fermentation in sugar cane bagasse. The highest activity of xylanases and celullases (P<0,01) was expressed by Trametes sp., with averages of 141.77UI·g^-1 DM and 1073.8UI·mg^-1 protein for xylanases, and 9.04UI·g^-1 DM and 69.16UI·mg^-1 protein for cellulases, without significant differences at 14 and 19 days. P. ostreatus and A. niger showed lower activities of these enzymes than Trametes sp., but they were similar among them and in both fermentation periods. The higher (P<0.01) laccasse activity was expressed by P. ostreatus with averages of 15.54UI·g^-1 DM and 128.75UI·mg^-1 protein at 14 days, higher (P<0.01) than at 19 days (11.75 UI·g^-1 DM and 102.88UI·mg^-1 protein). In Trametes sp. the laccasse activity was lower than in P. ostreatus and similar in both fermentation times. In A. niger the laccasse activity was lower (P<0.01) than Trametes sp. and P. ostreatus. The activity of fibrolytic enzymes in Trametes sp. EUM1 indicates a potential use for biotechnological applications.

Palabras clave : Enzimas Fibrolíticas ; Hongos ; Tiempo de Fermentación .