Abstract

BARRANCO FLORIDO, Esteban et al. b-n-acetylglucosaminidase production by Lecanicillium (Verticillium) lecanii ATCC 26854 by solid-state fermentation utilizing shrimp shell . INCI [online]. 2009, vol.34, n.5, pp.356-360. ISSN 0378-1844.

Lecanicillium (Verticillium) lecanii produced chitinases using shrimp shell as inducer. Maximum production of b-N-acetylglucosaminidase was measured at 80h. Enzyme stability was obtained at temperatures ranging from 30 to 40°C and maximum activity at 50°C, pH 6.0. Enzyme activity increased with Ba²⁺, Co²⁺, Fe³⁺ and Zn²⁺. Bioassays against the phytopathogenic fungus Oidium spp. showed mycelial and germination inhibition. SDS-PAGE electrophoresis of the partially purified extract revealed four bands of 70, 58, 45 and 31kDa and this extract showed activity of b-N-acetylglucosaminidase through zymogram analysis. Chitinases produced by L. lecanii are potentially useful against phytopathogenic fungi, insects and chitosan bioconversions.

Keywords : Antagonism; b-N-acetylglucosaminidase; Lecanicillium lecanii; Oidium ssp; Solid State Fermentation.