Abstract

ACEDO-FELIX, Evelia et al. Polyphasic typing of salmonella spp: Isolated from cantaloupe melon (cucumis melo) and coriander (coriandrum sativum) fields. INCI [online]. 2009, vol.34, n.6, pp.419-423. ISSN 0378-1844.

The serological and genetic diversity of Salmonella spp. strains isolated from Cantaloupe melon (Cucumis melo) and coriander (Coriandrum sativum) fields were evaluated. Biochemical test, serology, antibiotic resistance and PFGE were performed to identify isolated strains. Eleven contaminated samples from coriander fields were found, and seven from melon fields. The serogroups identified were mainly Salmonella anatum and S. give. Only one strain was resistant to tobramicyn. PFGE showed five electrophoretic profiles, in agreement with the identified serotypes and in the polyphasic analysis five clusters were observed (<35% similarity). It is necessary to implement good agricultural and production practices, since identified serogroups have been involved in epidemic outbreaks. Moreover, the polyphasic analysis showed genomic variability and also suggests that strains of S. give are clonal. It is not the same for S. anatum strains, which were isolated from different samples.

Keywords : Caracterización polifásica; Coriandrum sativum; Cucumis melo; Salmonella spp.