Resumo

SALCEDO-MORALES, Guadalupe et al. stablishment of a premeabilization protocol with detergents to increase betacianine production efficiency in a Beta vulgaris L. Cell cultures. INCI [online]. 2009, vol.34, n.9, pp.666-671. ISSN 0378-1844.

Red beet (Beta vulgaris L.) cell suspensions were permeabilized by means of four chemical detergent agents, Tween 20, 40 and 80, and Triton X-100^®, to evaluate the recovery of betacianins (BC). The permeabilizating agent was selected as a function of the quantity of BC released and the contact time. Betacianin concentration was measured using digital color image analysis. The results showed that 36% of betacianins was released using Triton X-100^® (0.7mM) during 10min; using these extraction conditions, the viability remained at 60-70%. This treatment allowed a second growing-cycle, as well as, an additional accumulation of betacianins.

Palavras-chave : Análisis de Imágenes; Betacianinas; Beta vulgaris; Cultivo de Células; Permeabilización.