Resumen

RODRIGUEZ, Y. et al. Detección de enfermedades virales afectando al pimentón en los municipios Iribarren, Jiménez y Torres del estado Lara, Venezuela, utilizando la técnica ELISA.. Rev. Fac. Agron. [online]. 2004, vol.21, n.2, pp.105-115. ISSN 0378-7818.

Sweet pepper is an important crop in Lara State, where farmers harvest about 50% of the national production. This crop has been affected in previous years, by the increase of a complex of apparently viral symptoms, mainly dwarfing, severe mosaic, leaf curl, fern leaf, blistering and leaf deformation. In order to get current information about which viruses are present, 85 samples in Iribarren, Jiménez and Torres counties of Lara state were collected and analyzed by ELISA technique with commercial kits against eight different viruses, some of them have been previously reported in the country affecting sweet pepper and other important solanaceous species. Several samples were inoculated on indicator plants and their particles were observed in a transmission electron microscope. The ELISA technique enabled the detection of following viruses: Cucumber mosaic Cucumovirus, CMV; Tobacco rattle tobravirus, TRV; Potato Y potyvirus, PVY; Tobacco etch potyvirus, TEV; Tobacco mosaic tobamovirus, TMV; Pepper mild mottle tobamovirus, PMMOV; Tomato ringspot nepovirus, ToRSV; and Tobacco ringspot nepovirus, TRSV. TRV, ToRSV and TRSV are detected in sweet pepper by first time in the country

Palabras clave : Sweet pepper; Capsicum annuum; virus; ELISA; detection.