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Revista de la Facultad de Agronomía
versión impresa ISSN 0378-7818
Resumen
DE MARCANO, Alcalá; VARGAS, N y PIRE, A. Effect of vegetables extracts and synthetics fungicides on the micelial growth of in vitro Sclerotium rolfsii and Thielaviopsis basicola. Rev. Fac. Agron. [online]. 2005, vol.22, n.4, pp.315-324. ISSN 0378-7818.
The antifungical effect of castor extracts Ricinus communis L.; sweet basil Ocimum basilicum L.; wild peppergrass Lepidium virginicum L.; garlic Allium sativum L.; neem Azadirachta indica A. Juss and fungicides benomyl (Benlate) and iprodione (Rovral) was proved on the mycelium growth in vitro of Sclerotium rolfsii and Thielaviopsis basicola. Sodium hypochlorite at 3% and distilled water were used to disinfect and to wash the vegetal parts. For the obtaining of juicy extracts, materials were macerated, the extract filtered with Whatman Nº1 paper and then sterilized with Millipore filters of 0.45 mµ. All tested extracts and fungicides showed some inhibit activity on the mycelial growth of Sclerotium rolfsii and Thielaviopsis basicola, in a papa dextrose (PD) mean, resulting in garlic a total inhibition of grow until 7 days. Benomyl and garlic reduced the grow of Thielaviopsis basicola virtually to cero during 21 days. Significant differences were found with iprodione (79%), benomyl (54.1%) and castor (8.3%) in relation to the witness. The number of sclerotium was lower in treatments with garlic, neem and benomyl compare to the witness (27.6, 49.3, 68.3 sclerotium vs 143.5 sclerotium). According to these results garlic might be considered as an alternative to be used in a handle strategy of these fungi.
Palabras clave : Vegetal extracts; fungicides; mycelial growth; Sclerotium rolfsii; Thielaviopsis basicola.