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Revista de la Facultad de Agronomía
versão impressa ISSN 0378-7818
Resumo
ALBANY, N et al. A methodology for the in vitro propagation of Aloe vera L.. Rev. Fac. Agron. [online]. 2006, vol.23, n.2, pp.215-224. ISSN 0378-7818.
Aloe (Aloe vera L.) is one of the economically important crops of the Liliaceae family. Vegetative propagation does not satisfy national demand being possible the mass production of aloe plants through in vitro propagation. An experiment was completed in order to evaluate the effect of different concentrations of NaClO (1, 2 and 3%) in the surface sterilization during the establishment stage (ES). In the multiplication stage (MS), the interaction of the culture media (semi-solid or liquid) with two different sectioning of the explants (transversally cut and transversally with partly lengthwise cuts) was evaluated, also in the acclimatization stage (AS) vitroplant survival was studied according to their size (small<5cm, medium 5-10 cm and large >10 cm). A completely randomized design was used to evaluate the variables: contamination percentage (C%) and mortality rate (M%) in the ES, multiplication coefficient (MC) and height of the explants (HE) in the MS and survival rate (S %) in the AS. Significant differences (P<0.05) were observed among NaClO concentrations being 3% the best (9.09%C). In the MS only the physical state of the culture medium rendered any statistical differences (P<0.01) for height of explants (HE) with 3.85 cm in liquid and 2.83 cm in semi-solid. No statistical differences were found in the AS for S%. All vitroplants adapted to ex-vitro conditions regardless their initial size.
Palavras-chave : Aloe vera L; establishment; multiplication; acclimatization.