Resumo

LEON, Graciela; CRUZ, Carlos  e  ROJAS, Lola. Production of Monoclonal antibodies with Anti B specificity used As A blood typing reagent. Invest. clín [online]. 2004, vol.45, n.2, pp.145-157. ISSN 0535-5133.

Abstract The purpose of this research was to produce murine monoclonal antibodies with anti B specificity to be used as reagents in red cells typing. Balb/c mice were immunized with substance B from saliva of a donor secreting B antigen, in order to obtain spleen cells or splenocytes producing antibodies with anti B specificity. These cells were fused with mouse myeloma cells P3-X63.Ag8.653 by using the PEG technique. After seeded, the hybridomas secreting the putative antibodies were cloned through the limiting dilution technique to obtain monoclonality. Afterwards, the antibodies were evaluated using immunological and immunochemical methods. BMS-4 clone secreting antibodies IgM-kappa with anti B specificity were obtained, which displayed high potency, excellent avidity, great sensibility and long time stability. We think that this clone can be used to produce a red cell typing reagent.

Palavras-chave : Monoclonal antibodies; specificity anti B; hybridomas.