Resumo

SANCHEZ-ROSALES, Milagros; OSORIO-MORALES, Sergio  e  DIAZ-ARAUJO, Felipe. Evaluation of the inmunohistochemical markers PCNA and MIB-1 in the diagnose of low grade lesions of the uterine cervix.. Invest. clín [online]. 2004, vol.45, n.3, pp.213-220. ISSN 0535-5133.

Abstract. A prospective and comparative study of 112 uterine cervix biopsies, received from hospitals of Maracaibo, Venezuela, with the diagnosis of cervical intraepithelial neoplasia I (CIN I) determined with the traditional Hematoxilin-Eosin (H-E) diagnostic staining, was performed to compare the inmunohistochemical markers PCNA and MIB-1 with the conventional H-E staining. The samples were classified with H-E, and 3 samples were excluded of the study with a different diagnostic from CIN I. After the inmunohistochemical study, 19 biopsies (17.4%) were classified as CIN II with PCNA and 18 (16.5%) with MIB-1. The analysis showed a significant difference (p < 0.01) between the H-E staining and the inmunomarkers. These results allow us to conclude that the inmunohistochemical evaluation of the proliferative activity is very useful to establish with certainty the diagnosis of preneoplasie lesions of uterine cervix and also represents and important parameter in the prognostic evaluation. 

Palavras-chave : Uterine cervix; PCNA; MIB-1; inmunohistochemical; cervical intraepithelial neoplasia .