Resumen

PREMOLI, Gloria; GALINDO-CASTRO, Ivan  y  RAMIREZ, José Luis. Sistema de detección y tipificación de Papilomavirus del tracto genital inferior: Hibridización in situ y confirmación con reacción en cadena de la polimerasa. Invest. clín [online]. 2005, vol.46, n.4, pp.369-379. ISSN 0535-5133.

We developed a simplified and non-isotopic in situ hybridization procedure for the detection of Human Papillomavirus (HPV) in routine Papanicolaou cervical smears. The assay involves one oligonucleotide (malignant probe) which recognizes high risk HPV 16 and 18, and another which detects HPV 6 and 11 (benign probe). We adapted the system to fulfill the requirements of gynecologists and cytologists, assimilating their protocols and simplifying the in situ hybridization assay. When we compared the detection levels reached by the in situ hybridization versus a ladder PCR assay in 156 clinical samples, original designed for this work, the kappa coefficient between both methods is 0.945, indicating a strong agreement between the ISH and the PCR assays

Palabras clave : Diagnostic; typing; HPV; in situ hybridization; polymerase chain reaction; lower genital tract.