Abstract

CHUAIRE-NOACK, Lilian et al. ß-Galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium. Invest. clín [online]. 2010, vol.51, n.3, pp.351-367. ISSN 0535-5133.

ß-galactosidase activity reflects the rate of cellular aging in vitro. Such activity was quantified at pH 6 in ovarian epithelial cells from 28 donors without a history of cancer, by the chemoluminiscent method. The cells were serially cultured until they achieved the state of permanent growth arrest. During the exponential growth phase, all cultures showed a similar pattern of growth and low ß-galactosidase activity. However, both in the onset of decrease replicative activity, as well as in the onset of the stationary phase, there was a significant rise in the enzyme activity. Our results showed that ß-galactosidase activity can be considered as a replicative senescence marker of the ovarian surface epithelium at pH 6.

Keywords : ß-galactosidase; replicative senescence; biomarker; ovarian epithelial cells.

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