Resumo

GUZMAN L, Militza et al. bla_TEM, bla_SHV y bla_CTX-M genes in extended-spectrum b-lactamases producing enterobacterias isolated from patients with hospital-acquired infections. Invest. clín [online]. 2013, vol.54, n.3, pp.235-245. ISSN 0535-5133.

The objective of the present investigation was to identify the bla_TEM, bla_SHV and bla_CTX-M genes on extended-spectrum b-lactamases (ESBL) producing Enterobacteriaceae from clinical isolates, collected between September and November 2005. In addition to third-generation cephalosporin resistance, the isolates also showed resistance to chloramphenicol (59.2%), amikacin (37.0%) and gentamicin (40.7%), and demonstrated sensitivity to imipenem and meropenem. Nine strains were capable of transferring third-generation cephalosporin resistance, as well as the production of ESBL. In the clinical isolates, the genes bla_SHV, bla_TEM and bla_CTX-M were detected, being more prevalent the types bla_TEM-1, bla_SHV-1, bla_SHV-5 bla_SHV-5-2a and bla_CTX-M-1; while in the trans-conjugated only bla_TEM-1, bla_SHV-5 y bla_SHV-5-2a were found. In total, seven types of genes were identified, five of which were codifying genes for ESBL-type enzymes. This demonstrates that in the hospital center, resistance to third-generation cephalosporin is mediated by several enzymes.

Palavras-chave : Plasmids; b-lactamases; gen bla_SHV; gen bla_CTX-M; gen bla_TEM; Resistance.

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