Resumo

ZAMBRANO, Edgar Armando; DE LA CRUZ, Henry Samuel  e  COITA, Blanca Elena. Description of an acidic peptidase, insensitive to classical inhibitors, in protein extracts of Trypanosoma cruzi, from a rural area of Venezuela, where Chagas disease is endemic. Invest. clín [online]. 2013, vol.54, n.3, pp.270-283. ISSN 0535-5133.

Through two peptidase assay methods, one in liquid-phase and another, in gel-phase (gel zymography), an acid peptidase was detected in protein crude extracts of epimastigotes of Trypanosoma cruzi, from a rural area of Venezuela where Chagas disease is endemic. The peptidase shows activity at a pH range between 2.0 and 2.9. Under the experimental conditions described, the acid peptidase was insensitive to usual concentrations of peptidase inhibitors of the types: serine, cysteine, aspartic and metallo-peptidases. Nevertheless, like porcine pepsin at pH 2.9, the peptidase was inhibited in the presence of 5mM DTT.

Palavras-chave : peptidase; peptidase inhibitors; Trypanosoma cruzi.

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